On fertile soils, a significant yield increase was observed only with S30 fertilization and only at nitrogen levels of N105 and N225. Applying fertilizer at an N ratio of 3.5:1 resulted in a significant increase in total amino acid content, while a lower S rate (7.5:1) showed only a trend towards increased amino acid content. When sulphur was applied in the form of ammonium sulphate, it significantly increased grain yield in two specific cases: during the regeneration of winter wheat vegetation and at the end of the crop tillering stage. Sulphur fertilizer also increased the number of grains per ear. Additionally, the protein content of the grain increased significantly only after the application of ammonium sulphate. Before applying sulphur, it is advisable to test the concentration of S-min in the soil. If the soil sulphur content is high, additional crop fertilization will not have a positive effect.